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VitriSafe - vitrification of oocytes and embryos

The “VitriSafe” is an embryo carrier device consisting of a large gutter in which a small quantity of CPs (< 1 µl) containing the blastocysts can be deposited. Before plunging the biological material into liquid nitrogen (LN2), the “VitriSafe” is completely inserted into a high security 0.3 ml straw (CBS, Cryo Bio System, France).

Welding of both edges of the outer protective straw took place before being plunged into LN2. This ensures a hermetic isolation of the sample against LN2. Under these conditions, the cooling rate decreased from > 20,000°C/min to ~ < 1,300°C/min.


med.techn. Abt. der Mobil-Tec Elektronik GmbH